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Firstly, the multiplex detection ability of ,ELISA, is seriously deficient because of the simplex ,enzyme,/substrate reaction, whereas a single marker, such as carcino-embryonic antigen (CEA), is often inadequate for the diagnosis of heterogeneous and complex diseases like cancer. 3,4 Secondly, most of the commercial ,ELISA kits, need targets in nanogram concentrations for naked-eye signal readout ...
January 2012 to November 2014, were ,selected, and enrolled in the experimental group, and 90 healthy subjects who under - went physical examination were enrolled in the control group. The expression level of miR-378e in serum was detected by reverse transcription-quantitative PCR and the expression of LI-cadherin in serum was detected by ,ELISA,.
human CD147 ,ELISA kit, (Beijing Lvyuan Bode Biotechnology Co., Ltd.), human MMP-9 ,ELISA kit, (,Shanghai, Zeye Biological Technology Co., Ltd.), human CRP ,ELISA kit, (,Shanghai, Chuan Qiu Biotechnology Co., Ltd.), and human IL-6 ,ELISA kit, (,Shanghai, Yubo Biological Technology Co., Ltd.). There were standard wells, blank wells, and sample wells.
A human serum ACE2 ,enzyme-linked immunosor-bent assay, (,ELISA,) ,kit,, produced by ,Shanghai, Bluegene Biotech Co., Ltd. (,Shanghai,, China), was used and tested by a Thermo Scientiﬁc Varionskon Flash (Thermo Fisher Scientiﬁc, Waltham, MA) in our study. The ,kit, was taken out from refrigerator and then incubated at room temperature (20–25°C) for ...
A new double-antigen sandwich-based ,enzyme-linked immunosorbent assay, (,ELISA,) for the detection of total antibodies (immunoglobulin G [IgG] and IgM) specific for hepatitis E virus (HEV) was developed by utilizing well-characterized recombinant protein ET2.1 and its peroxidase-labeled counterpart. Our study showed that the ,ELISA, detected all the positive patient samples ( n = 265) regardless of ...
14/10/2020, · Cell-free supernatants were assembled and used in the evaluation of oestrogen (E2) and progesterone (P4) production with an ,enzyme-linked immunosorbent assay, (,ELISA,) ,kit, (Zhenke, ,Shanghai,, China). Based on the ,kit, specifications, the absorbance at 450 nm with 50 μL supernatants was set using an Epoch microplate reader (Biotek, Winooski, USA).
On the basis of the specification of ,ELISA kit, (,Shanghai enzyme,-,linked, Biotechnology Co., Ltd., ,Shanghai,, China), seven standard wells were added with various concentration standards (100 μL) in turn, the blank wells were appended with 100 μL standard dilution solution, and the remaining wells were appended with 100 μL sample to be tested.
12/7/2016, · ,Abcam,’s Human ACE (CD143) in vitro ,ELISA, (,Enzyme-Linked Immunosorbent Assay,) ,kit, is designed for the accurate quantitative measurement of Human ACE (CD143) in cell culture supernatants, serum, plasma (heparin) and saliva. A ACE (CD143) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated.
Brucellosis is a serious zoonosis that occurs worldwide, and its diagnosis is typically based on the detection of antibodies against Brucella lipopolysaccharide (LPS). However, the specificity of the LPS-based test is compromised by cross-reactivity with Escherichia coli O157:H7 and Yersinia enterocolitica O:9. ,Also,, diagnosis based on the LPS test cannot differentiate between vaccinated and ...
15/2/2019, · 1. Introduction. ,Enzyme,-,linked, immunesorbent assay (,ELISA,), which was pioneered by Engvall and Perlman  and van Weeman and Schuurs  in 1971, makes the best of the highly specific recognition of antibody-antigen and the efficiently catalytic amplification of an ,enzyme,.At present, ,ELISA, is the most widely used mean of immunoassay [3,4], and has been extensively used in clinical …