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Mycoplasma detection, is difficult, sometimes impossible, when using traditional microbiological techniques. The current ,Mycoplasma, test is a 28-day culture test. In 2007, the European Pharmacopeia accepted nucleic acid tests, such as real-time PCR, as an alternative method for traditional ,Mycoplasma detection, after validation.

Kit, Specificity. This ,Mycoplasma, PCR ,Detection Kit, detects ,Mycoplasma, species simply, reliably, and rapidly. To detect the presence of these microorganisms, the assay uses the polymerase chain reaction (PCR) to amplify a target unique to a wide variety of mycoplasmas.

Kit, Specificity. This ,Mycoplasma, PCR ,Detection Kit, detects ,Mycoplasma, species simply, reliably, and rapidly. To detect the presence of these microorganisms, the assay uses the polymerase chain reaction (PCR) to amplify a target unique to a wide variety of mycoplasmas.

Mycoplasma Detection, * ,Mycoplasma,-positive culture was diluted into ,mycoplasma,-free culture medium to generate test samples with decreasing ,mycoplasma, concentrations. The PCR-based test method showed the highest ,detection, sensitivity which was up to 5,000-times higher than with a commonly used, well-established enzyme-based assay. 600,000 500,000

A qPCR ,kit, for ,detection of mycoplasma,. Takara Bio USA, Inc. (TBUSA, formerly known as Clontech Laboratories, Inc.) provides ,kits,, reagents, instruments, and services that help researchers explore questions about gene discovery, regulation, and function.

TransDetect ® PCR ,Mycoplasma Detection Kit, is designed to detect the presence ,of mycoplasma, contamination by PCR in biological materials, such as cell cultures or cell culture related materials. Highly specific primers have been designed to amplify a fragment of 16S rRNA coding DNA that is conserved across all commonly-known ,mycoplasma, species.

Materials and methods for ,mycoplasma detection, and elimination The maintenance of contamination-free cell cultures is fundamental to cell-based research. Among the most pervasive concerns is contamination with ,mycoplasma,, a genus of bacteria whose members are typically sub-micron in size and lacking a cell wall, making them difficult or impossible to detect by microscopy and resistant to …

The e-Myco™ ,Mycoplasma, PCR ,Detection Kit, (ver.2.0) consists of a set of primers designed to detect the presence ,of mycoplasma, and determine the species. This ,kit, can detect five common cell culture-infecting types ,of mycoplasma,. The species ,of mycoplasma, can be determined by sequencing the amplified products. 49 species can be detected.

1/8/2012, · The LookOut ® ,Mycoplasma detection kit, by Sigma-Aldrich is based on PCR, and claims that it can detect two ,mycoplasma, genomes in a single microliter of sample. The R&D Systems MycoProbe® ,Mycoplasma Detection Kits, are based on microarray hybridization, and include a high-throughput screening assay that the company claims is as sensitive as PCR.

Principle, of the Test Norgen’s MycoplasmaPCR ,Detection Kit, constituents a ready-to-use system for the isolation and ,detection of Mycoplasma, using end-point PCR. The ,kit, first allows for the isolation ,of Mycoplasma, DNA from the blood samples using spin-column chromatography.